Regulatory

Part:BBa_K319005:Design

Designed by: Afnan Azizi   Group: iGEM10_uOttawa   (2010-10-16)

yeast mid-length ADH1 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 93


Design Notes

There were three options for the length of the promoter - short, middle, and long. The middle length was chosen.


Source

The part was extracted from S. cerevisiae YPH500 strain using colony PCR protocols. It consists of 720 bp upstream of ADH1 gene. The primers were designed according to the genomic sequence found on [http://www.yeastgenome.org Saccharomyces Genome Database]. The design, extraction, and cloning the BioBrick part were performed by Afnan Azizi of 2010 uOttawa iGEM team.

References